• One limitation of using iPSCs is the inability to carry

  2018-10-22

  

  One limitation of using iPSCs is the inability to carry out studies under genetically defined conditions, leading to increased experimental variability. To overcome these obstacles, we used a zinc-finger-nuclease (ZFN)-mediated gene-editing technique (An et al., 2012; Corti et al., 2012; Li et al.,

• Knockout of Dnmta and Dnmt b in ESCs leads to

  2018-10-22

  

  Knockout of Dnmta3 and Dnmt3b in ESCs leads to progressive hypomethylation, supporting the notion that de novo activity is required to maintain 5mC in ESCs, possibly to offset persistent 5hmC conversion (Chen et al., 2003). However, importantly, Dnmt3a/b null ESCs only exhibited an ∼10%–20% reductio

• Hence we propose a model

  2018-10-22

  

  Hence, we propose a model in which transient HES5 activity in early mesoderm instructs the timing of cardiac fate specification while repressing the first wave of hematopoiesis. Thus in the absence of this signal, commitment to EryP is favored by default. Moreover, after a pulse of Hes5 instructing

• The following are the supplementary data related

  2018-10-20

  

  The following are the supplementary data related to this article. Acknowledgments This study was performed within the framework of CTMM, the Centre for Translational Molecular Medicine. We thank the AIRFORCE project for supporting this study (grant 03O-103). Introduction Pluripotency is defi

• In this regard several existing

  2018-10-20

  

  In this regard, several existing computational pathway analysis methods can potentially identify deregulated signaling pathways from high-throughput datasets (Hung, 2013; Khatri et al., 2012). In particular, due to huge progress in microarray and sequencing techniques, computational methods tend to

• Thus it appears that in the

  2018-10-20

  

  Thus, it appears that in the adult SVZ, in the absence or low levels of Noggin, BMP proteins expressed by astrocytes and NSC bind and activate BMPR, presumably BMPR-1A, activating its downstream SMAD proteins. Activated SMADs up-regulate the expression of the miRNA coding gene Mirg which encodes miR

• This study was approved by the Institutional

  2018-10-20

  

  This study was approved by the Institutional Stem Cell Research Oversight (ISCRO) committee in the Johns Hopkins University. Blood CD34+ mk-801 were obtained with an informed consent from the patient. Materials and methods Acknowledgments We thank the patient for providing blood sample from w

• Our studies employ a transgenic system with

  2018-10-20

  

  Our studies employ a transgenic system with H2BGFP expression controlled by an HSPC-specific human (hu) CD34 promoter (Radomska et al., 2002). In this Tet-off system, HSPCs continually incorporate H2BGFP until doxycycline (Dox) is administered (Schaniel and Moore, 2009). We have investigated the pro

• Finally it is also plausible that SIRT

  2018-10-20

  

  Finally, it is also plausible that SIRT1 deficiency may impair proper telomere DNA Damage DNA Repair Library in iPSCs through deregulation of telomere recombination in a telomerase-independent manner. Interestingly, recombination-mediated telomere elongation associated with the early stages of embry

• The dedifferentiation of lineage committed cells

  2018-10-20

  

  The dedifferentiation of lineage-committed Quinacrine Dihydrochloride into more primitive stem-like states during transplantation is similar to the cell-state transitions that occur during tumor initiation (Keller et al., 2010). For example, preceding the onset of basal cell carcinoma, adult tumor-

• As a potential platform to model HD cellular pathogenesis an

  2018-10-20

  

  As a potential platform to model HD cellular pathogenesis, an important goal of this study was to assess whether our cellular model recapitulates disease-associated pathologic events. We found that an increase of intranuclear inclusions and the accumulation of oligomeric, soluble, and insoluble mHTT

• It was recently shown that

  2018-10-20

  

  It was recently shown that hPSCs can be expanded as cell-only pterostilbene in serum-free suspension culture irrespective of matrix supplementation (Amit et al., 2011; Olmer et al., 2010; Singh et al., 2010), which is mandatory for conventional surface-attached propagation of hPSCs. In contrast to

• A variety of suitable murine xenograft models are currently

  2018-10-20

  

  A variety of suitable murine xenograft models are currently used in hematopoietic research, yet these animals require conditioning prior to experimentation. Given the increasingly restricted access to radiation sources, NBSGW mice are broadly enabling, allowing laboratories without access to radiati

• Although previous work showed that SOX is sufficient to conv

  2018-10-20

  

  Although previous work showed that SOX2 is sufficient to convert fibroblasts into NES+ multipotent NSCs under a unique culture condition (Ring et al., 2012), our genetic approach using Nes-CreER;Rosa-YFP mice failed to detect a stable NSC state during the in vivo reprogramming process. This differen

• br Experimental Procedures br Author Contributions br

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction Human embryonic stem cells (hESCs) are capable of unlimited self-renewal in culture while maintaining the potential to differentiate into any cell type present in the human body and thus provide researchers great o

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